It is used to enhance the fertilization phase of in vitro fertilization (IVF) by injecting a single sperm into a mature egg using micro-manipulators.
This procedure is an extension of the IVF and not a substitute. ICSI is indicated in the following cases when:
a- The sperm is too poor for IVF such as low count, poor movement (motility or progression) or high percentage of abnormal forms (morphology)
b- Previous failed or poor fertilisation during IVF. The sperm has been microsurgically recovered from the epididymis or testes.
c- The male partner is affected with HIV, Hepatitis B or C.
Following egg retrieval in the IVF cycle, each mature oocyte is inseminated with single sperm with micromanipulator unlike in IVF process where only the mixing of sperms and oocytes is done. The embryo transfer is done after 3-5 days.